Sentence examples for by using a pcr from inspiring English sources

Samples that yielded PCR products were confirmed by using a PCR assay incorporating the 120-M59´ and 120 807´ primer pair, which amplifies a 833-bp fragment of the ompB gene of Rickettsia, as previously described by Roux and Raoult (13 ).

PCR products were purified by using a PCR purification kit (ZYMO Research), and 150 ng of purified PCR products were applied for direct sequencing by capillary electrophoresis (Agowa, Berlin, Germany).

We have cloned rFTR1 by using a PCR approach relying on degenerate primers designed from the conserved regions of Saccharomyces cerevisiae high affinity iron permease.

DNA was recovered by using a PCR purification kit (Qiagen, Valcncia, CA) and then used for PCR or QRT- PCR (quantitative real time polymerase chain reaction).

Quality of cDNA was assessed by using a PCR control run with human β-actin.

As little as 1 genome could be detected by using a PCR assay (29 ).

Telomerase activity was determined based on the ability to produce telomere repeats by using a PCR-based TRAP assay and measuring the PCR products using an EIA-based assay.

Genomic DNA was genotyped by using a PCR-based procedure.

Here, we created and phenotypically analyzed segmentally haploidized strains, each harboring a deletion of one copy of approximately 100 300 kb of the left or right terminal region of 16 chromosomes in a diploid strain by using a PCR-mediated chromosomal deletion method.

By using a PCR-based approach, fragments of genes coding for H+-PPases in a number of protists, both free-living and parasites of animals and plants, that belong to diverse taxonomic groups (trypanosomatids, ciliates, apicomplexans, euglenoids, amoeboid mycetozoa, heterokonts) have been isolated.

Two different combinatorial TEVp substrate libraries, in the form of XNLXFXG and XNLXFXX, respectively, where X is any amino acid, were constructed by using a PCR-based strategy.