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To examine the apoptotic activity of T cells in vitro, T cells were obtained from the spleens of mice (several strains) involving CB-17 and gld (FasL deficient) and their C57/B6 backgrounds, by using a nylon fiber column (Wako Pure Chemical Industries).
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The assay called spoligotyping (spacer oligonucleotide typing) screens 43 spacers by hybridization using a nylon membrane[29].
The DNA samples were digested with XhoI, separated by 0.7% agar gel electrophoresis and blotted using a nylon membrane (Hybond-N+, GE Healthcare Life Sciences) by the capillary transfer method.
Clean glass bakeware using a nylon scrubber.
Avoid using a nylon that has holes.
was mounted to the suspended ceiling above the patient bed to the head side by using a pair of nylon cords and a microphone holder.
Baboon serum samples were blotted onto a nylon membrane by using a slot-blot manifold according to the protocol described by Zaaiger et al., which has a detection limit of 2.5 × 10 HBV genomes/mL (17 ).
NP specimens were obtained by using a mini tip flocked with Nylon fiber swab (code 518CS01; Copan, Brescia, Italy).
Denatured DNA (200 ng) was transferred onto a nylon membrane (Roche) by using a Bio Dot apparatus (Bio-Rad) according to manufacturer's specifications.
For TIL isolation, freshly isolated tumours from HNC patients were minced into small pieces, which then were transferred to a cell strainer (70 μm Nylon) and mechanically separated by using a syringe plunge.
Agarose gels used for Southern transfer were denatured for 45 min in denaturing solution (0.5 mol/L NaOH, 1.5 mol/L NaCl) before being neutralized in 0.5 mol/L Tris-HCl, pH7.5, 1.5 mol/L NaCl for 30 min. DNA was then transferred to Hybond (Amersham, Buckinghamshire, England, UK) nylon membrane by vacuum by using a custom-made Southern blotting apparatus.
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