The quality of total RNA was assessed using a bioanalyzer (Agilent 2100; Agilent Technologies) and RNA was quantified by using a ND-1000 Nanodrop spectrophotometer.
RNA was quantified at OD260 nm by using a ND-1000 spectrophotometer (Nanodrop Technology, Wilmington, Delaware USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, Santa Clara, California USA) with RNA 6000 nano labchip kit (Agilent Technologies).
Here, we overcame these issues by using a neodymium:yttrium-aluminum-perovskite (Nd YAP) laser with a wavelength that allows selective activation of nociceptive afferents even in the glabrous skin [ 5] and by telescoping the laser beam down to a spot size of 1.3 mm.
DNA concentrations were quantified by using a NanoDrop ND-1000 UV-Vis Spectrophotometer (NanoDrop Technologies, USA) according to the manufacturer's protocol.
RNA was quantified by using a Nanodrop ND-100 spectrophotometer (Labtech, Uckfield, East Sussex, UK) and assessed for purity by UV absorbance measurements at 260 and 280 nm.
The quantity and purity of the total RNAs were determined by using a NanoDrop ND-1000 spectrophotometer (NanoDrop ND-Thermo Fisher Scientific, Wilmington, DE, USA) and their integrity by using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, http://www.agilent.com/).
The quantity and purity of the total RNAs were determined by using a NanoDrop ND-1000 spectrophotometer (NanoDrop ND-Thermo Fisher Scientific, Wilmington, DE, USA) and their integrity determined by using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, http://www.agilent.com).
The amplified RNA (aRNA) was generated and quantified by using a NanoDrop ND-1000 spectrophotometer (NanoDrop ND-Thermo Fisher Scientific, Wilmington, DE, USA), and biotinylated nucleotide analog was incorporated during in vitro transcription step.
An experimental setup was designed by using a Nd YAG laser operating at 1064 nm (200 mJ per pulse, 10 Hz) and an Echelle spectrometer with ICCD detector.
An experimental setup was designed by using a Nd YAG laser operating at 1064 nm and an Echelle spectrometer with ICCD detector.
The performance of laser-induced breakdown spectrometry (LIBS) for the determination of Ba, Cd, Cr and Pb in toys has been evaluated by using a Nd YAG laser operating at 1064 nm and an Echelle spectrometer with intensified charge-coupled device detector.
