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Finally, we demonstrate the utility of terminator selection for metabolic engineering by using a mutant xylose isomerase gene as a proof-of-concept.
These data show that antibodies against cholera toxin can be induced in vivo by high level expression of a non toxic mutant, or by using a mutant with residual ADP-ribosyltransferase activity.
Here we studied this question by using a mutant strain of Synechocystis 6803 cyanobacterium, which lacks the slr0228 gene that encodes one of the FtsH homologues.
Enhancing or inhibiting this TB reaction pharmacologically or by using a mutant virus enhanced or inhibited transmission, respectively, confirming its requirement for efficient virus-acquisition.
Specificity was controlled by using a mutant (hereafter termed 3R) in which three hydrophobic residues in the TMD were changed to arginine.
What is the effect of blocking AKAP/RIIα interactions (e.g., using peptide competition or by using a mutant construct that does not bind RIIα) on the FRET signal for each of the three RIIα proteins?
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To compensate for this, the formation rate was calculated using Equation 3. The formation rate of the stem deletion mutation was verified by a second method, in which mDEL1 mutants were detected selectively by using a purR mutant tester strain (TT26169).
We then examined whether deficiencies in the CIA machinery would have an effect on Pri2 protein levels by using a ts mutant of TAH18.
For the toggle switch, LacI was provided constitutively; the level of active repressor was controlled by using a lacY mutant and varying the concentration of IPTG.
The sensitivity of the Tango assay is improved by using a β2AR mutant (β2/V2R) that has its carboxy-terminal tail replaced with that of the vasopressin 2 receptor.
In this work it is shown that this yield on light can be improved by 51% by using a starchless mutant, and a similar improvement seems realistic for the areal productivity in outdoor cultivation.
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