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Test virus suspension was prepared by infecting RAW 264.7 cells (ATCC TIB-71) with MNV (Berlin 06/06/DE isolate S99, kindly provided by the Robert Koch-institute in Berlin) by using a multiplicity of infection of 1.
By spinoculating cells with Env-deficient, VSVg-pseudotyped virus, we ensured a synchronous, single-round infection, and by using a multiplicity of infection (MOI) sufficient to infect >90% of cells, we minimized confounding effects from bystander (uninfected) cells.
Monkeypox virus (Zaire strain) and vaccinia virus (Western Reserve strain) were grown in BSC40 cells by using a multiplicity of infection of 0.1 and harvested at 48 h postinfection.
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(16) Optimal multiplicity of infection (MOI) was determined by using Ad-EGFP and by measuring the percentage of fluorescent cells 24 hours later.
Virus stocks were generated by infection of confluent BHK-21 cells using a multiplicity of infection (MOI) of 1.
Virus stocks were generated by infection of confluent BHK-21 cells with this virus using a multiplicity of infection (MOI) of 0.1.
By evaluating the results of (2) and (3) with the multiplicity cut, we confirmed that a large number of the soft components were removed using a multiplicity cut.
For decades, most Arab rulers have denied their people these rights, using a multiplicity of laws and regulations.
Infections were performed using a multiplicity of infection of 100 bacteria per cell.
Inference on model parameters using a multiplicity of data sources is challenging.
On day 1, medium was removed and virus added using a multiplicity of infection of 0.3.
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