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The identification results obtained by ribotyping corresponded to those collected by using a multiplex PCR protocol specifically designed for C. jejuni and C. coli detection.
Aflatoxigenic Aspergillus flavus isolates always show, by using a multiplex PCR-system, four DNA fragments specific for aflR, nor-1, ver-1, and omt-A genes.
Samples also underwent NAT for other respiratory viruses by using a multiplex PCR.
blaCTX-M genes were screened by using a multiplex PCR assay (30 ).
Tube coagulase-positive isolates were speciated by using a multiplex PCR specific for the thermonuclease (nuc) gene (7 ).
The mosquito and swine specimens were subjected to viral RNA detection by using a multiplex reverse transcription PCR (RT-PCR).
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IL-1β and TNF-α in human plasma, mouse plasma, and mouse lung homogenates were detected by immunofluorescence using a multiplex panel assay (R&D Systems, Minneapolis, MN).
The presence of four groups of OXA-carbapenemase genes (namely blaOXA-23-like, blaOXA-24-like, blaOXA-51-like and blaOXA-58-like) was detected by PCR using a multiplex assay as previously described [ 19, 20].
Considering that cytokines and chemokines are important in muscle regeneration, we determined the presence of 18 different cytokines/chemokines secreted in TA muscles at different time points after CTX-induced damage (0, 4, and 12 h, and 1 d and 3 d) by using a Luminex multiplex platform (Table S2).
Theoretically, 99% coverage should be obtained by using a higher multiplex custom assay of 1,536 SNP.
SCC mec typing was performed on all MRSA isolates by using a described multiplex real-time PCR (20 ).
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