In the present work, by using a calcein accumulation method in combination with immunorecognition and drug-resistance studies, a new method is described for the quantitative estimation of the expression and function of the multidrug transporter.
The potency was confirmed by several assays (including cytotoxic drug sensitivity MTT assay, enhancement of drug uptake Vybrant™ multidrug resistance assay, inhibition of drug efflux assay, and mechanisms of NSC23925 inhibition by Pgp-Glo assay) using a panel of multidrug resistant cell lines with different degrees of drug resistance and Pgp1 expression.
Using a mouse lung-infection model caused by a multidrug resistant Pseudomonas aeruginosa mucoid strain isolated from a cystic fibrosis patient, we evaluated bacteriophage treatments.
The bactericidal effects of BATT1 and BATT2 on multidrug-resistant nosocomial pathogens were assessed using a suspension-inhibition test as recommended by the European Committee for Standardization (CEN) with or without bovine serum albumin (BSA) 0.3 g/100 ml [ 13].
Current recommendations for prophylaxis include using a macrolide once a week, while treatment usually requires a multidrug regimen.
While we previously showed by using inside out vesicles that the multidrug BmrC/BmrD transporter catalyzed the transport of several drugs [31], not every substrate was found here to stimulate the ATPase activity of the transporter.
PubMed and Medline databases were searched for reviews and original reports based on primary studies by using the keywords tuberculosis, Mycobacterium tuberculosis, multidrug-resistant, and Africa.
Struski et al. identified the chromosomal loci associated with non-P-glycoprotein-mediated multidrug resistance to the topoisomerase II inhibitor in a lung adenocarcinoma cell line by using comparative genomic hybridization.
Several K. pneumoniae international clones associated with multidrug resistance or hypervirulence were described by using MLST (4 – 8, 21 ).
The antimalarial assay was carried out at Virginia Tech against the multidrug-resistant strain Dd2 of P. falciparum by using the SYBR Green I-based plate assay as previously reported [19].
