Sentence examples for by using a microtome from inspiring English sources

Histological sections (8 µm) were prepared by using a microtome Richard-Allan Scientific Micromm) with a low profile microtome blade (Richard-Allan), straightened on Fisherbrand ColorFrost Plus microscope slides with 0.5% gelatin and allowed to dry for 2 d at 40°C on the top of a slide warmer.

Serial sections obtained by using a microtome were pasted onto microscope slides with egg white.

Thin sections (4 5 μm thickness) were prepared by using a microtome and stained with hematoxylin-eosin.

Paraffin blocks were sectioned at 6 μm by using a microtome (HM355; Microm/Thermo Fisher Scientific, Waltham, MA, USA).

After this time, all of the tissues were embedded in paraffin for being posterior cross-sectioned in thin 4  μM slices, by using a microtome (Microtome Olympus Cut 4060, USA).

Coronal sections (5-µm) were cut through the striatum (at 1.2 mm caudal to the bregma) and the SNc (from −4.5 to −6.2 mm caudal to the bregma) by using a sledge microtome (Wetzlar, Germany).

Next , 4μm thick sections were collected from each scaffold by using a Polycut microtome (Reichert-Jung, Heidelberg, Germany).

Successive sections of the spinal cord tissues were sliced at a thickness of 30 µm by using a cryo-microtome and adhered onto gelatin-pretreated slides.

Serial coronal sections (30 μm) were cut from the rostal to caudal edge of the brain tissues containing the tumours by using a cryo-microtome.

A desirable aspect ratio could have been achieved using a microtome as performed by Lake et al. (2010); however, this involves freezing the tendon which can impact on tendon mechanical properties (Clavert et al. 2001), thus we chose to test unfrozen tissue.

After paraffin infiltration, the tissues were sectioned using a microtome, stained with hematoxylin and eosin (H&E) and analyzed by light microscopy.