The optical density (OD) at 450 nm was measured by using a Microplate Reader (Thermo Scientific, VaripsKan Flash).
The amount of resorufin produced proportional to the number of viable cells was quantified by using a microplate reader (described earlier) equipped with a 560 nm excitation/590 nm emission filter set.
The absorbance at 560 nm was measured by using a microplate reader (Biorad, USA).
The absorbance was measured at 570 nm by using a microplate reader.
The absorbance was measured at 492 nm by using a microplate reader (Perkin Elmer Envision Multilabel reader).
After 5 min room temperature (RT) incubation, absorbance at 734 nm was measured by using a microplate reader (Biorad, Hercules, CA, USA).
The fluorescence intensity of sample was measured five times with a 20-μs integration time by using a microplate reader and BCA protein assay kit.
After 30-min incubation, absorbance at 570 nm was read by using a microplate reader (Molecular Devices, Orleans Drive, Sunnyvale, CA, USA).
After the mixture was incubated at 37 °C for 10 min, the absorbance was measured at 475 nm by using a microplate reader (Molecular Devices, Sunnyvale, CA, USA), which allowed the calculation of the tyrosinase inhibition (Kim et al. 2010).
Samples at each concentration (0.01 2.0 mM) were dissolved in ethanol, reacted with a 0.1 M DPPH reagent for 10 min at 25 °C, and monitored at 517 nm by using a microplate reader (Molecular Devices, Sunnyvale, CA, USA).
