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The aminated magnetic nanoparticles were then collected by using a magnet and were then washed two times with toluene and three times with methanol and were then dried in an oven at 50°C and were stored in a vacuum desiccator.
Briefly, 1 mL of infected Vero cells supernatant was recovered after 11 days of infection (first observation of the cytopathic effect), ultracentrifugated (250 000 rpm at 4°C for 3 h in a Beckman SW41 rotor ultracentrifuge) and then incubated with 10 µg of magnetic beads for 10 min. Magnetic beads were then recovered by using a magnet and the preparation was analyzed by western blot.
The magnetic and nonmagnetic particles were separated by using a magnet.
For example, you can observe an electromagnetic field by using a magnet to move a paperclip.
Moreover, the catalyst can be easily recovered by using a magnet.
The obtained Ag/Au SSCN composite nanospheres demonstrated promising catalytic properties, which could also be recycled very easily by using a magnet.
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The culicoids were collected during October 14 16 by using a Mosquito Magnet Independence trap (Mosquito Magnet, Lititz, PA, USA) baited with carbon dioxide and octenol.
The product GA-MNP were recovered magnetically from the reaction mixture by using a permanent magnet with a surface magnetization of 6,000 G, then washed three times with 100 mL of distilled water, and finally dried in an air oven at 50°C for 24 h and stored in a stoppered bottle for further use.
It is convenient to collect the dried particles when they are not coated by surfactant using a magnet.
After that, the products were collected, separated by sorting using a magnet, and then washed several times with deionized water.
10 The obtained fraction of PBMCs was incubated with bead-labeled anti-CD4, anti-CD8, anti-CD14, or anti-CD15 antibodies (Miltenyi, Cologne, Germany), followed by isolation using a magnet.
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