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The program enables mothers who do not deliver at a CCBB cord blood collection site to donate their baby's cord blood by using a kit.
The HRP reaction was revealed by using a kit for chemiluminescence (Super Signal West Dura Extended Substrate, 34075, PIERCE) and measured by a phosphor imager (Biorad).
Serum calcium was determined by using a kit (DICA-500 QuantiChrom™) from BioAssay Systems (Hayward, CA).
Determination of homocysteine was carried out by using a kit based on immunoassay (Abbott Laboratories, Ltd., Pakistan).
For ChIP-DSL assays, both input (5% of total DNA) and IP (antibody-enriched DNA) were randomly biotinylated by using a kit (Vector Laboratories) according to the manufacturer's instructions.
Cyclic GMP was measured by using a kit from Cayman Chemical Ann Arborr, MI).
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Bisulfite modification of genomic DNA was carried out by using a methylation kit (EZ DNA methylation kit; Zymo Research, Orange, CA).
Systemic IL-10 levels were measured by ELISA using a kit from BD Biosciences (Cat 555157) following manufacturer instructions.
Serum osteocalcin was measured by ELISA using a kit from SenXiong Technologies Incorporated (Shanghai, China).
Plasma insulin and Aβ42 levels were measured by ELISA using a kit (Bluegene Biotech Co., Shanghai, China).
Aldo was determined by RIA using a kit from Demeditec Diagnostics GmbH, Kiel, Germany.
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