From each flock, 10 pooled cloacal swab samples are obtained at slaughter and analyzed for Campylobacter spp. by using a genus-specific PCR (34 ), and results have been collected in the national surveillance database.
All samples were screened by using a Rickettsia genus-specific qRT-PCR targeting the gltA gene and an R. felis-specific qRT-PCR targeting the bioB gene (4 ).
All other samples were tested by using a Plasmodium-genus specific qRT-PCR targeting the Cox-1 gene found in all Plasmodium species; the primers Psp_15.F (5′-AGGAACTCGACTGGCCTACA-3′) and Psp_16.R (5′-CCAGCGACAGCGGTTATACT-3′) and the (6FAM-5′-CGAACGCTTTTAACGCCTGACATGG-3′TAMRA) probe were used.
If one genus comes under another genus, it will be possible, in some cases incumbent, to prove that a property belongs to a genus by using a theorem from another science.
Our previous investigations by means of immunofluorescence using a genus-specific antiserum against chlamydial LPS and scanning as well as transmission electron microscopy showed infection of the alveolar parenchyma and the bronchioles by Chlamydia spp. in patients having undergone lung volume reduction surgery for advanced pulmonary emphysema [ 16, 17].
E. coli was identified by the lactose and indole tests, and other enterobacteria were identified to the species or genus level by using a microtube identification system (API 20 E®; bioMérieux S.A., Marcy-l'Etoile, France).
Similarly, no Mycobacterium genus band was detected by using a PCR-hybridization method (Common Mycobacteria line probe assay, Hain Lifesciences GmbH, Nehren, Germany) (3 ).
Malaria species was identified by using a nested PCR with Plasmodium genus specific outer primers (M18SF0: 5′-CCATTAATCAAGAACGAAAGTTAAGG-3′ and M18SR0c: 5′-TGTTGTTGCCTTAAACTTCCTTG-3′) derived from small subunit rRNA for a primary PCR (7 ).
Initially believed to be related to the Plainview point classification, the new type was termed "Plainview Golondrina" by Johnson, who used a genus-species approach for the naming.
end{aligned} Moreover, when F satisfies an additional symmetric condition, by using the genus theory, we also obtained that system (1.1) has infinitely many solutions.
Motivated by the above works described, the object of this paper is to study the existence and multiplicity solutions for a class of sublinear fourth-order elliptic equation of Kirchhoff type by using the genus properties in critical theory.
