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In this study, we fabricated composite tablets consisting of two components, a drug and a filler, by using a fused deposition modeling-type 3D printer.
The two analytes were separated within 6 min with the optimized conditions: 50 mM borate buffer, 25 mM SDS pH 7.8, 35 °C, at 50 mbar 4 s injection and 30 kV by using a fused silica capillary (72 cm effective length, 50 μm i.d).
The glass coverslip induced band in the region 1000 2000 cm−1 was overcome by using a fused silica coverslip which leaves only a peak at 798 cm−1 in the Raman spectrum.
We confirmed that a non-fused PCNA complex assembled from PCNA1, PCNA2 and PCNA3 also supports coordinated PolB1, Fen1 and Lig1 maturation activity and thus our observations are not affected by using a fused heterotrimeric PCNA (Supplementary Figure S4).
The developed method allowed the use of small volumes of sample and solvents and rapid separation by using a fused core column (only 2.2 min were needed).
A fluorescent sensor that can monitor levels of extracellular ammonium has been made by using a fused green fluorescent protein to detect conformational changes in ammonium transport proteins.
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The ratio of the three stereoisomers of 2,3-BD was analyzed by GC (Agilent GC6820) using a fused silica capillary column (Supelco Beta DEXTM 120, inside diameter 0.25 mm; length 30 m) [ 40].
A CUBE 3D (from 3D System) printer is used to print out a PLA prosthesis from the STL file using a fused deposition model by means of a 1,75 mm filament at a 260 °C extruder temperature (Fig. 6).
The resultant esters were redissolved in hexane and separated by GCMS using a fused-silica capillary column (Rtx-20; Restek, Bellefonte, PA).
The resulting FAME were then analysed by GC using a fused-silica capillary column (CP-Sil 88; 100 m × 0.25 mm i.d., 0.20 mm film thickness; Chrompack, Varian Inc., Walnut Creek, CA, USA), equipped with a flame ionisation detector, as described by Bessa et al.[ 65].
The resulting FAME were then analysed by GC using a fused-silica capillary column (CP-Sil 88; 100 m × 0.25 mm i.d., 0.20 mm film thickness; Chrompack, Varian Inc., Walnut Creek, CA, USA), equipped with a flame ionisation detector, as described by Bessa et al.[ 9].
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