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Each stage of the impactor was calibrated using a gravimetric or counting method and the fully assembled impactor was evaluated by using a fluorometric method.
We determined cell viability by using a fluorometric resazurin reduction method (CellTiter-Blue, Promega, Madison, WI) following the manufacturer's instructions.
The cytotoxic activity of the CGs was measured by using a fluorometric microculture cytotoxicity assay (FMCA) as previously described [23], [24].
Plasma vitamin C concentrations were measured within 1 wk of sampling by using a fluorometric assay.
The intensity was measured by using a fluorometric plate reader (PerkinElmer) at 485 nm excitation and 535 nm emission.
Plasma nitrite/nitrate (NO) levels were measured by using a fluorometric assay kit (Cayman Chemical, Ann Arbor, MI, USA).
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By using a simple fluorometric assay, we were able to measure CFD levels in severe septic patients.
We next investigated the underlying mechanism of apoptosis induced by AV-65 using a fluorometric protease assay.
The cell growth by DNA content was determined using a fluorometric DNA assay as described previously [ 15, 28].
The assay was performed according to the manufacturer's instructions (Apo-ONE Homogeneous Caspase 3/7 Assay, Promega) and the release of the fluorogenic moiety by activated caspases was measured by reading plates at 405 nm using a fluorometric plate reader.
The sensitivity and resistance of each cell line to TOC, three other kinase inhibitors, and the cytotoxic agents VBL or CCNU were determined by measuring relative viable cell number using a fluorometric bioreductive assay (Alamar Blue, Promega; Madison, WI).
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