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The fluorescence intensity was measured by using a fluorescent spectrophotometer.
The fluorescence intensity of the supernatant was quantified by using a fluorescent spectrophotometer (Infinite M200, TECAN, Switzerland) at an excitation wavelength of 470 nm. S. aureus bacteria (strain MRSA252) were cultured on tryptic soy broth (TSB) agar overnight at 37 °C.
To remove the excess dye and stain, the nucleus for quantitative analysis, samples were washed with PBS and resuspended in PBS containing DAPI. Green fluorescence and the respective DAPI images were captured by using a fluorescent microscope.
Relative fluorescence intensity was recorded over time 3-1200 min) by using a fluorescent plate reader (Thermo, Appliskan) at an excitation wavelength of 485 nm and emission was measured at a wavelength of 530 nm, and fluorescent images were also obtained by using a fluorescence microscopy (ZEISS, Axiovert 200 M).
The concentrations of MPs in different tissues were determined by using a fluorescent spectrophotometer with excitation = 418 nm and emission = 518 nm.
We develop a system for measuring rotational diffusion coefficients by using a fluorescent probe to label a particle.
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GFP can be replaced with a cell surface marker normally absent in CHO cells, such as CD20, in order to isolate high producers by FACS using a fluorescent antibody against the marker [ 11].
After centrifugating the homogenates, the supernatants were collected, derivatized with ethylenediamine to detect L-dopa or with diphenylethylenediamine to detect catecholamines, and then analyzed by HPLC using a fluorescent detector.
The assay was performed by using a specific fluorescent caspase-3 substrate (DEVD-AFC) that on hydrolysis produces a green fluorescent product.
Therefore, in this paper, extensive experiments were performed by using a miniature fluorescent detection system; the effects of the parameters including the concentration and the volume of fluorescent dye solution, the coefficient of Stern Volmer plot, and the volume ratio of the fluorescent dye solution and the chloride solution were studied; and a modified Stern Volmer Equation was obtained.
For each tumor two hybridizations were performed by using a reversal fluorescent dye.
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