After ammonia had been consumed, the cells were de-repressed for 3 h and subsequently harvested by using a flow-through centrifugal harvester (Cepa, Lahr/Schwarzwald, Germany).
Because the process of bilayer formation can be done within the spectrometer by using a flow-through system, we could easily monitor this process by the infrared absorptions of the lipids, and obtained the kinetics and the difference spectrum of the immobilization.
The final radiochemical purity was measured by instant thin layer-silica gel chromatography (ITLC-SG; Pall Life Sciences, Ann Arbor, MI, USA) developed in 100 mM sodium citrate, pH 5.0 or by size-exclusion HPLC using a flow-through radioactivity detector (FSA; PerkinElmer).
CO2 production was measured using a flow-through respirometry system.
An attempt to compare the two flow regimes present in batch and semi-continuous apparatuses was done by Liu and Wyman that used a flow-through system under stopped flow condition to simulate the batch behavior [ 20].
Selective electrocatalytic dehalogenation of 2,4-D in aqueous solution containing MeOH and tetraalkylammonium salt was directly performed under constant current electrolysis by using a membrane-separated flow-through cell with permeable cathodes made of palladium-loaded carbon materials.
The electrochemical hydrodechlorination of 4-chlorobiphenyl (4-MCB) in aqueous solution containing methanol (MeOH), sodium acetate, acetic acid and bromide of hexadecyltrimethylammonium (CTAB) was investigated under constant current electrolysis at ambient temperature by using a membrane-separated flow-through cell operated in batch-recycle modes.
The presence of citrullinated epitopes was detected by using a pool of 26 purified anti-CCP2 IgG eluate fractions from SF and the corresponding flow-through IgG pool.
Therefore, we can comprehend the organization in real systems for which a random walker is not a good proxy for flow through the system, by using a different model of flow or by directly measuring the real flow.
First, the dissolution profiles were compared by using a paddle method (PD) and a flow-through cell method (FT) under three flow rates.
By using a genetic method of attenuating charge flow through ASIC channels, we reveal here that fear conditioning is impaired.
