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Fractions were applied onto a nitrocellulose filter by using a dot blot apparatus.
DNA macro-arrays on nylon membranes (Hybond N, Amersham Biosciences) were performed by using a dot blot assay according to Hänninen et al. [ 37] with some modifications.
Immunoglobulin (Ig) G antibodies to SARS-CoV were detected by using a dot blot enzyme-linked immunosorbent assay (ELISA) using a culture- derived, heat-inactivated virus antigen (E-E Ooi, unpub. data) at a serum dilution of 1 100.
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Cells were gated on the Forward versus Side Scatter plot to eliminate debris, and then single cells were gated by using a dot-plot showing the pulse height versus pulse area of the DAPI channel (see Supporting Information Fig. S1).
In addition, the app will now push you to visit other areas by using a red dot on various sections and tabs to let you know when there's activity.
Denatured DNA (200 ng) was transferred onto a nylon membrane (Roche) by using a Bio Dot apparatus (Bio-Rad) according to manufacturer's specifications.
For dot-blotting, samples were directly applied onto a Hybond ECL nitrocellulose membrane (GE Lifesciencs) by using a Bio-Dot microfiltration apparatus (BioRad, Hercules, CA, USA) as specified by the manufacturer.
Currently, the detection of anti-SRP autoantibodies is based on indirect immunofluorescence on HEp-2 cells, followed by confirmation using a dot-blot test or a quantitative addressable laser bead immunoassay (ALBIA) [ 16, 17].
Also, by using a reporter quantum dot, this nanobarcode platform can monitor and quantify gene expression level with improved speed and sensitivity.
In this work, by using a novel quantum dots-based "off-on" fluorescent lateral flow test strip (LFTS), the authors describe a rapid and sensitive biosensor for point-of-care testing of GSH.
To investigate the role of pericardial ROS on cardiac function, we decreased ROS levels specifically in PCs by using a PC-specific Dot-GAL4 driver to overexpress the H2O2-degrading enzyme, catalase (Zámocký et al., 2012).
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