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Here, we identify this repressor which we call muscle growth regulator (MGR)—by using a DNA protein interaction screen based on quantitative mass spectrometry.
Parasite DNA templates were isolated from blood by using a DNA isolation Kit (High Pure PCR Template Preparation Kit, Boehringer Mannheim, Germany).
An artifactual background due to DNA repair appeared in our DNA replication assays with time at 37°C, which we could abolish by using a DNA repair inhibitor (see Data S1, Fig. S4 and Fig. S5).
Recently, forensic scientists in California used a genetic analysis procedure called "familial searching" or "kinship searching" to help the police identify a suspect in the "Grim Sleeper" serial murder case — and they did so by using a DNA sample collected for another purpose from the suspect's own son.
Now, researchers have shown they can predict the deadliness of one type of cancer by using a DNA chip to determine which genes it activates.
When Elizabeth Warren took the bait of her political rival by using a DNA test to "prove" her claims, she endorsed a way of thinking about race, DNA and Native ancestry that reinforces white supremacy.
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Therefore, it is clear that TQ mediated effects on telomerase and telomere length were significantly reduced in the absence of DNA-PKcs achieitherither by using a DNA-PKcs deficient cell type or by functional inhibition of its kinase activity in DNA-PKcs proficient cells.
10 µg of RNA was treated with DNase I by using a DNA-free kit (Ambion) and used as the templates for RT-PCR analysis.
PCR products were analyzed by using a DNA-500 kit on MCE-202 MultiNA (SHMADZU, Kyoto, Japan).
We tried to validate the specificity of these interactions by using a DNA-binding deficient form of AbdA.
Contaminating genomic DNA was removed from the isolated RNA by using a DNA-free kit (Ambion, Huntingdon, UK) and total RNA content and purity were determined spectrophotometrically at 260 and 280 nm.
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