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The drugs were suspended in vehicle immediately before use and administrated orally (10 ml/kg) by using a disposable 1 ml syringe and gavage needle (0.9 mm diameter).
The samples were weighed, and lyophilized samples were homogenized to powder by using a disposable pestle.
Before harvesting the fat, the site was injected with carbocaine and adrenaline at very high dilution (40 ml of 2% carbocaine and 1 vial of 1 mg/ml adrenaline in 500 mL cold saline solution) by using a disposable 17G blunt cannula connected to a luer-lock 60-cm3 syringe.
Mice were inoculated in the right parietal lobe by using a disposable 25-gauge hypodermic needle.
Frozen lung tissues from each dog were thawed and homogenized in lysis buffer containing β-mercaptoethanol by using a disposable tissue grinder (Kendall, Lifeline Medical Inc., Danbury, CT, USA).
Skin samples were collected by using a disposable scalpel over an area of ≈0.2 × 0.2 × 0.2 cm from inter ocular frontal area of the head and soaked at 37°C in saline solution for 1 h.
Similar(54)
After an initial incubation of 30 min at 65°C, the softened spine material was disrupted by hand using a disposable micropestle.
With the assistance of a qualified nurse high vaginal swab (HVS) was taken (by using a sterile disposable speculum) at the emergency room of the Obstetrics/Gynaecology Department.
Caries was diagnosed at cavitation level mainly by visual inspection using a disposable dental mirror attached to an intra-oral LED light [ 17].
In the present study, a new, convenient and efficient production system by P. pastoris using a disposable plastic culture-bag was developed, which requires neither special ingredients in the culture medium nor expensive equipment such as a jar fermenter.
Following treatment, cells were washed in ice-cold PBS and harvested by mechanical dislodging using a disposable cell scraper (Sarstedt Inc). in the presence of cell lysis buffer (NEBS), supplemented with phenylmethylsulfonylfluoride for 5 min.
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