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After reaching confluence, MSC were induced to differentiate by using a differentiation kit (R&D Systems, Minneapolis, MN).
By using a differentiation protocol in which cells are exposed to FBS for the first 2 weeks and defined exogenous factors for the subsequent 4 weeks (Fig. 2I), the absolute number of rhodopsin-positive cells was increased significantly (∼82,000±18,000/well) (Fig. 3).
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Furthermore, the colorimetric responses were visualized quantitative by using a color-differentiation map prepared form converted RGB (red, green and blue) values.
Fat differentiation of ASCs was induced by using a basic fat differentiation medium with or without IBMX (3-isobutyl-1-methylxanthine).
Definite and random disturbances are also considered when comparing the present method with that by using a technique of numerical differentiation.
In this study, we have utilized a more homogeneous population of cells by using a sophisticated in vitro differentiation system in order to minimize problems associated with the use of mixed-cell populations.
We undertook a detailed behavioral analysis of self-other attribution in a 67-year-old male patient with behavioral variant frontotemporal dementia (bvFTD) due to the C9ORF72 expansion by using a novel paradigm requiring differentiation of the effects of self- and non-self-generated actions.
"When you consider the other alternatives, it is so far unclear to me how we could maintain differentiation" by using a third-party operating system, Mr. Elop said.
We therefore sought for possible physical clusters (pCs) among the 1,282 transcripts that varied in abundance during differentiation by using a modified Smith-Waterman algorithm [15].
By using a modified protocol for ESC differentiation to IPCCs derived from Blyszczuk et al [1], [16] (Supplemental Figure S1), we now assess the early immune events that follow transplantation of IPCCs derived from ESF 122 ESC (H2k) or adult pancreatic islets isolated from CBA mice (H2k) [2], [13] [13] (Supplemental Figure S2).
PCR fingerprinting profiles showed polymorphisms within each of the 3 species, allowing for a clear differentiation, by using a "cut-off point" of >97% similarity.
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