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The number of RyR clusters were then measured for the same volume by using a detection algorithm [9] and expressed per unit volume of f-actin.
Briefly, trypsinized Caco-2 cells were collected, including floating apoptotic cells and the cells spontaneously detached during washing procedure, and annexin V-FITC and PI co-stained by using a detection kit from Medical & Biological Laboratories Co , Ltd ,aka-ku Nagoya Japan, accordiNagoyathe manufacturer's instructions.
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Mycoplasmas in CM and cell lysates were detected by using a mycoplasma detection kit (e-Myco, iNtRON, Sungnam, Korea) and M. arginini-detecting PCR using the following primer sequences: Forward: 5′-GATTCCGTTGTGAAAGGAGC-3′, Reverse: 5′-TCAAGCTTTCGCTC ATTGTG-3′.
Proteins were detected by using a chemiluminescence detection system from Roche.
After washing, proteins in the immunoprecipitated complexes were separated by 10% SDS-PAGE and detected by using a chemiluminescence detection system (Amersham Biosciences) and captured on the Super RX X-ray films (Fuji Film).
Rendered path [19] was proposed to solve the problem in irregular regions by using a hole detection method [22], which detects nodes at the boundaries of holes.
The watermark detection is carried out on the received signal by using a standard detection statistic method rho=frac{1}{L}boldsymbol{z}^{T}boldsymbol{u} (2).
Furthermore, the gain achieved by using a multistatic detection algorithm is also analysed.
These background changes can be strongly reduced by using a signal detection in the near-infrared (NIR) range.
In other words, the gain achieved by using a multichannel detection scheme is larger for simulated data with respect to real ones.
We developed a one-step, real-time PCR assay for rhinovirus by using a sequence detection system (Applied Biosystems; Foster City, CA).
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