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TUNEL staining was performed by using a 'Dead End' fluorometric TUNEL kit (Roche, Upper Bavaria, Germany) as per manufacturer's protocol.
Like the modified JC-1 assay, this assay identifies lysed cells by using a dead cell-specific nuclear stain, in this case PI.
An alternative method to identify target genes for distal Candidate Functional SNPs is to modulate the chromatin state of the element using ZFPs or TALEs fused to a chromatin modifying domain or by using a "dead" Cas9 that has no nuclease activity (dCas9) fused to a chromatin modifying domain; such engineered systems are termed "epigenetic toggle switches".
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The new compact force transducer was evaluated in its upright orientation by using a dead-weight type force standard machine and in the inverted orientation by using a force comparator in which an existing massive tuning-fork type force transducer was temporarily adopted as a reference.
Dr Wang told me, "You're more likely to be struck by lightning than vote illegally," by, say, using a dead person's identity to cast a ballot. .
We investigated the role of PI3K-Akt pathway in TGFβ-stimulated myofibroblast differentiation using LY294004, a specific PI3K activity inhibitor, and by using a kinase-dead (KD-Akt) construct of Akt.
The role of Akt2 in vivo was confirmed by using a kinase-dead (inactive) Akt2 mutant K181M-Akt22), which significantly reduced not only metastases but also tumour growth [ 1].
Las Ramblas is pretty intense, it was there that I once saw a tourist getting attacked by a homeless guy using a dead pigeon as a weapon.
ADCC activity was assayed by using a method to monitor dead cells by incubating cells with Calcein-AM, which labels viable cells.
In that light, it would make sense to increase the number of pairs in the DC design that touch upon dead and also to increase the power per pair as this approach would ensure that future studies conducted by using a DC model incorporating dead will be consistent for the whole multi-country dataset.
This is accomplished by using a threshold: cells are declared dead if they fall below this threshold, and live otherwise.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com