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Cell viability was evaluated before and after HCl administration by using a cytotoxicity assay (Roche GmbH, Mannheim, Germany).
LDH assay was carried out by using a cytotoxicity detection kit (Tox-7, Sigma-Aldrich) according to the manufacturer's protocol.
Lactate dehydrogenase (LDH) was measured as an indicator of cell death by using a cytotoxicity detection kit (Roche; Applied Science, Barcelona, Spain).
The sensitivity of MCF-7CC, MCF-7TXT and MCF-7DOX cells to various drugs was determined by using a cytotoxicity assay based on the Vybrant MTT Cell Proliferation Assay following drug treatment for 24 h or 72 h (data not shown).
Because DT production by toxigenic C. ulcerans is reported to be substantially lower than that usually seen in toxigenic C. diphtheriae (8 ) as was the case in the study patient, it is difficult to estimate the pathogenic contribution of these low amounts of DT detected only in vitro by using a cytotoxicity assay, but not by immunologic precipitation for this patient in vivo.
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Cell membrane integrity was assayed by LDH assay using a Cytotoxicity Detection Kit according to the manufacturer's instructions (Roche Diagnostics, Indianapolis, IN).
Cytotoxicity was determined by measuring LDH release using a Cytotoxicity Detection KitPLUS (LDH) (Roche) according to the manufacturer's instructions.
After 48 hours cell viability was assessed by measuring lactate dehydrogenase release using a cytotoxicity detection kit (Boehringer-Mannheim).
Cell viability was estimated by the LDH leakage method using a cytotoxicity detection kit (Roche) according to the manufacturer's instructions.
Cytotoxic effects were analyzed by measurement of lactate dehydrogenase release using a cytotoxicity detection kit (Roche, Basel, Switzerland).
The complement-dependent cytotoxicity (CDC) and antibody-dependent cellular cytotoxicity (ADCC) activities of anti-TFRC were measured by a lactate dehydrogenase (LDH -releasing assay using a cytotoxicity detection kit (Roche, IndianapoLDH -releasing
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