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It can be resolved by using a curve fitting procedure: one is located at 530.3 eV and the other one is located at 532.4 eV.
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DP polarograms were analyzed by using a curve-fitting procedure and equations given in the literature.
The 50% inhibitory concentrations (IC50s) were obtained by using a curve-fitting model with standard logistic regression.
The inverted monochrome image was adjusted by using a "Curves" tool until the background was removed and each chromosome of the spread became fuzzy-edged.
All assays were performed in quadruplicate and readings averaged and plotted against imatinib concentration as a best fit sigmoidal curve by using a nonlinear curve-fitting algorithm (Origin 7.0, Origin Lab, Northhampton, MA).
Tone curve alignment is a technique to remove the brightness difference as much as possible by using a tone curve determined automatically and optimally.
Concentrations were correlated with absorption values by using a calibration curve constructed using known concentrations of QE.
Phosphate concentration was determined by using a calibration curve made with KH2PO4 in the range 10 70 μM.
The amount of 4-nitrophenol released was calculated by using a standard curve of 4-nitrophenol concentration versus absorbance.
Hence, the interpolation is established by using a polynomial curve fitting that is based on linear model estimator.
The amount of H2O2 was calculated by using a standard curve prepared with known concentrations of H2O2.
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