Sentence examples for by using a conjugated from inspiring English sources
Immunohistochemical localization of antibodies to GFAP (1 500 GA-5 clone, mouse, Sigma), GLT-1 (1:5000, rabbit, Frontier Sciences) and Huntingtin 2B4 (1 600, mouse, Chemicon) was performed by using a conjugated second antibody method.
The T-spline control points are initialized from their correspondences in the B-spline patches, which are refined by using a conjugate gradient method.
In details, water molecules and counterions were first minimized for 250 steps by using a steepest descent algorithm (SD) and for 750 steps by using a conjugate gradient algorithm (CG), while keeping Gli1ZF/DNA frozen.
Cells were pulsed for 1 h with BrdU at final concentration of 1 mM and BrdU-positive cells were detected by using a FITC conjugated anti-BrdU antibody (Abcam).
The convergence is accelerated by using a preconditioned conjugate gradient method where the preconditioner takes advantage of the structured nature of the underlying mesh.
A computational procedure is designed to solve the discretized linear system at each time step by using a preconditioned conjugate gradient method.
Bound antibody is detected by PCR using a conjugate of anti-human IgG covalently coupled to an oligonucleotide.
31 As a next step, we explored protein peptide conjugations by using a DIBAC-FLAG conjugate as a model peptide (see the Supporting Information).
Reactions were visualized by using a suitable secondary antibody conjugated to horseradish peroxidase (Dako) in blocking buffer at room temperature for 2 hours and an enhanced chemiluminescence system (Pierce).
After washes with PBS supplemented with 0.2% I-Block (w/v) and 0.1% Tween 20, immunoreactivity was revealed by using a specific horseradish peroxidase conjugated anti-IgG secondary antibody and the enhanced chemiluminescence system (Supersignal West Dura, ThermoFisher Scientific).
By using a GRP78-targeting peptide conjugated to a PEGylated gold nanorod, photothermal damage could be induced after the application of light.
