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The use of confocal Raman microscopy has been reported as a way to prevent this [29] and Lorenzetti et al. [30] reported on its usefulness to determine the dyes on historic dyed cotton yarns by using a confocal condition 2 3 µm lower than the fibre surface.
The networks thus obtained are examined by using a confocal laser microscope as well as an atomic force microscope.
After being washed with PBS, the cells were imaged by using a confocal laser scanning microscope (SP8, Leica) equipped with a white-light continuum laser.
BSA-Rhod release profiles from single microspheres in solution and within the scaffold were assessed by using a confocal laser scanning microscopy (CLSM -assisted method.
Measurement of liquid-film thickness by using a confocal laser scanning microscopy gives information for evaluation on the heat transfer rate.
Gazzaley developed a method for measuring changes in the concentration of NMDA receptors in neurons from monkey and rat brains by using a confocal laser scanning microscope and sophisticated computer software.
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The images were captured by using an confocal microscope (LSM-700, Carl Zeiss, Jena, Germany) and the ZEN software (ver. 5,5,0,375, Carl Zeiss), which was designed for acquisition and processing of confocal images.
Additionally, wound healing assays were filmed by videomicroscopy, using a confocal microscope (TCS-SP-2-AOBS-UV, Leica).
PI Synthase expression was analysed in AMOL and HSC-2 cells treated with ST (10 μg/ml) for 48 h by immunocytochemistry using a confocal microscope.
The deposition of complement components (P, fH, C3 and MAC) on PTECs was observed by IF using a confocal microscope (Olympus Viewer 1000, Olympus, Tokyo, Japan), and quantification of the depositions was evaluated by FV 10 ASW 2.1 imaging software (Olympus).
Confocal imaging was performed by using a Nikon confocal microscope C1, (Kangawa, Japan) fitted with Ar, He/Ne, and blue diode lasers.
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