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The total flavonoid concentration was determined by using a colorimetric method with minor modification (Bao et al. 2005).
The assay was carried out by using a colorimetric, commercial kit (Calbiochem-Novabiochem Corporation, USA) according to the manufacturer instructions.
We have also investigated this phenomenon by measuring the activity of caspases by using a colorimetric caspases kit that measures the activity of caspases-3 in the samples.
Measurement of NO activity by using a colorimetric NO assay kit showed that Shh overexpression was associated with a concomitant increase in NO production in ShhMSCs (Figure 3C).
Then the cells were harvested and caspase 8 activity was measured by using a colorimetric ApoAlert Caspase-8 Assay Kit (colormetric) from Clonetech Laboratories, Inc. (Mountain View, CA) according to manufacturer's instructions.
The nitric oxide (NO) content was measured by using a colorimetric assay kit (BioVision, CA, USA).
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HCN was analysed by passing the whole mainstream smoke stream through two impingers of sodium hydroxide solution, followed by quantification using a colorimetric reaction and a continuous flow analyser system.
These changes can be easily analyzed by using a simple colorimetric assay system.
HDAC activity was measured by using a HDAC Colorimetric Assay Kit (MBL international, Woburn, MA).
VFL effect on growth inhibition was measured by using a MTT colorimetric cell viability assay.
Plasma C-reactive protein was measured by using a commercial colorimetric immunoassay (DimensionCardioPhase high-sensitivity C-reactive protein method; Siemens Healthcare Diagnostics Ltd).
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