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The reaction was run in a final volume of 2 mL of reaction buffer (0.5 M Na phosphate, pH 7.3) containing 0.1 mL of 3 mM EDTA, 0.1 mL of enzyme extract and 0.1 mL of 3 mM H2O2 for 5 min. Catalase activity was calculated by using a coefficient of absorbance of 0.036 mM−1 cmM−1
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Weak geochemical contrasts between the involved lithotypes minimize the assignment variances by using a fuzzy coefficient of 1.3.
By knowing this information, the method is modifying the SVs by using a correction coefficient, ζ: ζ = max Σ L L A ^ max Σ L L A (2).
MLVA9-Utrecht profiles were clustered with Bionumerics software (version 5.1) by using a categorical coefficient and a graphing method called minimum spanning tree [14].
MLVA profiles were clustered in the BioNumerics version 6.1 software by using a categorical coefficient and visualized by using the minimum spanning tree method.
The MLVA and MLST profiles were clustered with the Bionumerics software by using a categorical coefficient and a graphing method called minimum spanning tree [ 20].
Plots from each diffraction pattern were linearly scaled using a coefficient determined by least-squares fitting of the continuous equatorial diffraction data between 0.03 and 0.20 Å–1 (∼33–4 Å).
By multiplying the signal log ratio using a coefficient obtained from the probability value that took account of signals in neighbouring probes, its linearity was markedly improved (r = 0.94).
MDA concentrations were calculated by using an extinction coefficient of 155 mM−155m−155
The amount of TBARS was calculated by using an extinction coefficient of 155 mM-1 cm-1.
The level of protein carbonyl contents was calculated by using an absorption coefficient of 22,000 M-1 cm-1.
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