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Osteopontin (OPN) was detected by using a chromogenic substrate for the secondary antibody according to the kit manufacturer's instructions.
By using a chromogenic media-based test after 24 h of incubation, the isolation costs can be reduced, because of the shorter test delay and the higher specificity, but results in fewer infections averted.
Bound antibodies against PA were detected by using a chromogenic peroxidase substrate (tetramethylbenzidine).
The formed phosphate could be quantified by using a chromogenic reaction allowing spectrophotometric detection of the degree of conversion.
Similar data were also obtained by using a chromogenic method that employs succinylated casein as a substrate (QuantiCleave™ Peptidase Assay kit, Pierce).
Analysis of the HER-2 oncogene amplification was conducted by using a chromogenic in situ hybridization (CISH) method as described previously [ 27].
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Thus, the presence of endotoxin in the andrographolide preparation was checked, by using a quantitative chromogenic LAL assay.
Levels of aFXa activity were determined by using a validated chromogenic assay kit (COAMATIC Heparin; Chromogenix, Instrumentation Laboratory Company, Lexington, KE, USA) with the substrate S-2732, and the apparatus (STA-R Evolution; Diagnostica Stago, Asnières, France).
As a consequence, a less detrimental effect for prothrombin activation was observed with prothrombinase assembled with factor Va6A as demonstrated by our assay using a chromogenic substrate to assess α-thrombin generation.
At end-point, G-quadruplexes are forced to fold from PCR-generated duplex DNA and then are used to colorimetrically report the successful occurrence of PCR by assaying their peroxidase activity using a chromogenic substrate.
PK function levels were performed by a microtiter, amidolytic assay using a chromogenic substrate, S-2302 (Pro-Phe-Arg p-nitroanilide; Chromogenix, Moindal, Sweden), as described previously [ 27].
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