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We designed the unit switch by using a cell-based design method.
By using a cell-based assay designed to find small molecules with the ability to reactivate an epigenetically silenced locus and turn on its transcription, we have identified 19 validated novel potential transcriptional modulators.
In our study, from a chemical library consisting of 4000 chemically diverse compounds we firstly identified Z86 as a novel Wnt/ β-catenin signaling inhibitor that belongs to β-carboline structure-type compound by using a cell-based luciferase reporter assay system.
Based on previous reports showing that activin A and prostaglandins promote cell motility [ 22, 24- 26], we assessed the motility of vHMECs by using a cell-wounding assay.
To confirm the tumor repressor role of ERβ in the modulation of MMe cell growth, we expressed ERβ in the constitutively ERs-negative MSTO-211H MMe cell line, by using a pCXN2 based plasmid expressing ERβ.
The invasive potential of cancer cells was determined by using a model based on co-culture with fibroblasts, as previously described [ 25].
The IC50 of individual cell lines was calculated by using a linear regression based on the MTT assay results.
The optimum conditions for the EC process by using the present cell based on minimum initial hexavalent chromium concentration, energy consumption and operating cost were 100 mg of Cr VI /l, 0.55 A, 1.5 g of sodium chloride/land pH of 1.
NSCLC cell proliferation was assessed by using an assay based on cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases (Roche, Indianapolis, IN) as reported earlier [ 22].
We used a cell cultured based assay system.
We investigated the cytosolic proteins produced by irradiated tumor cells by using a quantitative proteomic approach based on stable isotope labeling by amino acids in cell culture.
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