Antimicrobial activities of the compounds were determined by using a broth microdilution method performed in sterile 96-well microplates.
Subculture and phenotypic antimicrobial susceptibility testing was performed by using a broth microdilution method approved by the Clinical and Laboratory Standards Institute at the University of Alabama at Birmingham.
Susceptibilities of the isolates were tested for penicillin, oxacillin, tetracycline, erythromycin, clindamycin, trimethoprim, chloramphenicol, vancomycin, gentamicin and ciprofloxacin by using a broth microdilution method (VetMIC™, SVA, Sweden) according to the existing standards [ 23, 24].
Testing for antimicrobial susceptibility was performed by using a broth microdilution panel (PML Microbiologicals, Wilsonville, OR, USA) containing the following 11 antimicrobial agents: ciprofloxacin, gentamicin, trimethoprim/sulfamethoxazole, clindamycin, tetracycline, erythromycin, rifampin, linezolid, mupirocin, vancomycin, and oxacillin.
On the basis of MICs of antimicrobial agents determined by using a broth microdilution method (5 ), the outbreak strains were found to be resistant to both extended-spectrum cephalosporins (cefotaxime, MIC >32 μg/mL) and fluoroquinolones (ciprofloxacin, MIC >8 μg/mL).
The vancomycin MIC of the above mentioned bacteria was determined based on the 0.5 McFarland standards, by using a microdilution broth and the Etest method.
Susceptibility of C159/11 and pCT transconjugants to a panel of antimicrobial drugs (ampicillin, cefotaxime, cefoxitin, chloramphenicol, ciprofloxacin, naladixic acid, streptomycin, and tetracycline) was determined by using a microtiter broth double dilution method (www.bsac.org.uk/_db/_documents/Chapter_2_Determination_of_MICs_2006.pdf).uk/_db/_documents/Chapter_2_Determination_of_MICs_2006.pdf
You can match the stock to the dish by using a beef-based broth or stock, although using chicken stock would is universal for braising any type of meat, and might add a nice complexity to your braised beef.
Antimicrobial activity was estimated by minimum inhibitory concentration (MIC) determinations using a broth microdilution procedure basically according to a guideline of the Clinical Laboratory Standards Institute (14).
In this study, we aimed to clarify the effect on S. aureus detection by using an enrichment broth prior to plating compared to direct plating.
The excised lymph node and neck abscess grew atypical mycobacteria, which were initially isolated by using an automated broth culture system (MGIT; Becton Dickinson, Sparks, MD, USA) after 4 weeks of incubation.
