By using a big-sized and very short outlet tube, the two injecting streams could impinge intensely within the limited chamber of the T-junction to achieve a quick mixing at the micro-scale.
By using a bigger PSD, a bigger object can be placed on it and be scanned.
Therefore, these enzymes together with the other lipases which were shown to be able to hydrolyze thin P 3HB-co-92 mol% 4HB) films were selected to further investigate the effect of enzyme concentration on hydrolysis sP 3HB-co-92ties by using a bigger range of enzyme concentrations.
Nucleotide sequences were determined by using a Big-Dye terminator cycle sequencing kit and ABI Prism 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA).
Sequencing reactions of the target fragments were performed in both directions by 'primer walking' using a Big Dye Terminator Cycle-Sequencing Kit (Applied Biosystems), and overlapping fragments were assembled to complete the sequence of the entire genome.
For each sample, the amplicons of the expected sizes were purified and sequenced using forward and reverse primers by cycle sequencing using a Big Dye Terminator version 1.1 Cycle Sequencing kit (Applied Biosystems, CA, USA) and an ABI PRISM 3130 sequencing device or sent for outsource sequencing (Primm).
To detect genetic alterations in K-ras and BRAF, we analysed the point mutations of codons 12 and 13 of the K-ras gene and the mutation of the exon 15 codon 600 of the BRAF gene by direct sequencing using a Big Dye Terminator v3.1, Cycle Sequencing kit, and an ABI Genetic Analyzer 3100.
PCR-fragments of 16S rRNA genes were sequenced by Sanger's method using a Big Dye Terminator v.3.1 reagent kit (Applied Biosystems, Inc., USA) on an ABI PRIZM 3730 system (Applied Biosystems, Inc., USA) according to standard instructions from the manufacturer.
Cycle sequencing reactions for each of the long PCR products were performed in both directions by the 'primer walking' method using a Big Dye Terminator Cycle-Sequencing Kit (Applied Biosystems).
