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Furthermore, for in vivo transdifferentiation, this method can be applied to translocate ribosomes to a targeted area by using a bacterial vehicle47,48.
The objective of this study was to evaluate the sealing ability of AH Plus, Epiphany, Acroseal, Endofill, and Polifil after active lateral condensation technique, by using a bacterial test, during 64 days.
Four X chromosomes also were seen by using a bacterial artificial chromosome clone encompassing the X inactive-specific transcript (XIST) locus as a probe (data not shown).
Secondly, we prepared a recombinant protein for CapZα-1 by using a bacterial expression system and further citrullinated the recombinant CapZα-1 by using PAD.
Now, Quan et al. have tackled this challenge by using a bacterial assay that they had developed previously and which links the correct folding of a test protein to cell survival and growth in the presence of an antibiotic.
By using a bacterial methyltransferase to add methyl groups to accessible, nucleosome-free, promoter regions at GpC (not CpG) dinucleotides in a single bisulfite sequencing experiment, native CpG methylation and nucleosome mapping can simultaneously be performed on the same DNA molecule.
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CLDN3 promoter luciferase reporter plasmids were constructed by PCR using a bacterial artificial chromosome clone as a template (AC093168, RP11-148M21/DH10B; Invitrogen) and the primers are listed in supplementary Table S2 (available at Carcinogenesis Online).
The chromosomal DNA was extracted from each strain by using a GenElute Bacterial Genomic DNA kit (Sigma-Aldrich, St . Louis MO, USA), and the DNA was used for PCR templates; the PCR conditions were as described (3 ).
Recombinant Cre expressing MHV-68 virus was constructed by E/T recombination using a bacterial artificial chromosome (BAC) containing the entire MHV-68 genome (kindly provided by Dr. U. Koszinowski, Ludwig-Maximilians-Munchen, Munich)[22].
Cell proliferation assays were performed by direct counting using a bacterial counting chamber (Hausser Scientific Partnership, PA, USA) and differential interference contrast microscopy.
Positive samples were verified by using a pan-bacterial PCR specific for the 16S rRNA gene (Technical Appendix).
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