The plasmids pUG6 and pUG66 were isolated from E. coli cells by using a commercial miniprep kit (Qiagen, Hilden, Germany).
Plasmids were isolated from one ml overnight cultures grown under selection using a miniprep kit (Qiagen) and quantified by OD260 readings.
Genomic DNA was extracted from mature leaves using a miniprep protocol [ 74] modified by the addition of a CTAB extraction [ 75]. A. thaliana leaves were harvested, hand washed and then ground to a fine powder in liquid nitrogen.
High quality plasmid was first prepared from 200 ml overnight culture of cells by standard alkaline lysis and phenol-chloroform extraction protocols, then further purified using a miniprep Kit (Qiagen, Valencia, USA).
Genomic DNA was extracted from lymphocyte samples using a Miniprep column system (GenEluteTM Mammalian Genomic DNA Miniprep Kit by Sigma, St . Louis MO, USA).
Plasmid DNA was purified using a miniprep kit (Qiagen), and the intended product was verified by DNA sequencing (UC Berkeley DNA Sequencing Facility).
The plasmid DNA mixture was extracted from the cell pellets using a miniprep kit.
Positive clones were grown in LB medium, and the plasmids were isolated using a miniprep alkaline lysis method [45].
Highly purified plasmid DNAs containing ILK, ILKK220A, or α-parvin were then prepared using a miniprep DNA purification kit (QIAGEN).
Plasmids were purified using a miniprep plasmid extraction kit (Qiagen).
