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The results were evaluated by using SmartCycler software (Cepheid).
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Real-time PCR was carried out by using SmartCycler® II (Cepheid, Sunnyvale, CA, USA) under the following conditions: initial denaturation at 95°C for 10 min, 50 cycles of 95°C for 30 s, 62°C for 60 s with fluorescence reading (set to FAM, which allows optical excitation at 480 nm and measurement at 520 nm) at the end of each cycle.
A one-step multiplex real-time reverse transcriptase-polymerase chain reaction (RT-PCR) using SmartCycler technology and TaqMan probes was developed for detection and typing of bovine viral diarrhea viruses (BVDV).
The threshold of the growth curve (CT) was set at a value of 30 using the SmartCycler software.
Quantification was performed by using ImageJ software.
Image analysis was performed by using PDQuest software.
Data were analyzed by using ScÅtter software, and the ab initio envelope structures were reconstructed by using DAMMIF/DAMMIN software.
Numerical example solved by using Matlab software.
Statistics were performed by using Microsoft Excel software.
MCSA was determined by using ImageJ software.
Analysis was performed by using R software.
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