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RNA was extracted from rotavirus-positive stool samples by using RNaid kits (MP Biomedicals/Qbiogene, Solon, OH, USA).
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RNA was extracted onto glass beads by using a RNAid kit (Bio101, Carlsbad, CA) according to a modified protocol (7).
RNA extracted by using the RNaid Kit (Qbiogene, Carlsbad, CA, USA) was reverse transcribed by PCR and amplified by using SuperScript One-Step RT-PCR with platinum Taq kit (Invitrogen, San Diego, CA, USA) with primers targeting the small, medium, and large segments (adapted from [ 11 ]).
Total RNA was extracted from human blood samples and rodent lung samples by using the RNaid (PLUS) Kit (BIO 101 Inc., La Jolla, CA) as described elsewhere (24 ).
Other parameters were determined by using commercial kits.
MMP-1 production and type 1 procollagen content were determined by using ELISA kits.
Glucose and glycerol concentrations in medium were measured by using the assay kits.
Supernatant medium on days 6 was collected for measurement of glucose and FFA by using glucose assay kit and FFA detection kit, respectively.
Content of FAS was measured by using the kit, intracellular FAS level was normalized to the content of total protein measured by using the protein assay kit.
Supernatant was collected for assessment of TG concentration by using the kit.
DNA was purified by using E.Z.N.A. Blood DNA kit 11 (Omega Bio-TEK).
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