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After determination of RNA concentrations by measuring the absorbance at 260/280 nm 0.5 μg RNA as template was reversely transcribed to cDNA by using QuantiTect Reverse Transcription kit (QIANGEN, Alabama, USA).
cDNA was generated from the RNA by using QuantiTect reverse transcription kit (Qiagen, Venlo, the Netherlands).
cDNA synthesis of RNA was carried out by using Quantitect Reverse Transcription kit (Qiagen, Hilden, Germany) and run with TaqMan Array Assay-on-Demand probes (Applied Biosystems, Life Technologies Corporation, Carlsbad, CA, USA).
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cDNA was obtained by transcribing 1 µg of total RNA using QuantiTect reverse transcription components (Qiagen).
Reverse transcription was performed using QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany).
First-strand cDNA was synthesized using QuantiTect reverse transcriptase (Qiagen).
Subsequently, cDNA was synthesized from 0.5 1 µg RNA using Quantitect Reverse Transcriptase kit.
Briefly, 1 µg of RNA was reverse-transcribed (RT) to synthesize cDNA using Quantitect Reverse Transcription Kit (Qiagen, Canada).
cDNA was generated using a mix of oligo dT and random primers using QuantiTect Reverse Transcription Kit (Qiagen).
Total RNA samples were converted to cDNA using Quantitect Reverse Transcription Kit (Qiagen) with removal of genomic DNA contamination.
DNase-treated total RNA was reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen).
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