Sentence examples for by using PrimeScript reverse from inspiring English sources

The cDNA was synthesized with 3.0 μg of total RNA by using PrimeScript Reverse Transcriptase (TAKARA).

mRNA expression was detected by reverse transcription of 500 ng of total RNA into cDNA using PrimeScript reverse transcriptase and random primers (TaKaRa, Otsu, Shiga, Japan), followed by real-time PCR with SYBR Green Applied BiosystemsSYBR Green Applied Biosystems

All total RNA were treated with RNase-free DNase I (Promega) and cDNA were synthesized using PrimeScript Reverse Transcriptase (TaKaRa) under the condition recommended by the manufacturer procedure.

First-strand cDNA was synthesized using PrimeScript reverse transcriptase (TaKaRa).

For PrimeScript 5′-RACE: Reverse transcription was performed on 1 μg of total RNA using PrimeScript Reverse Transcriptase (Takara), according to manufacturer's instructions.

First-strand cDNA synthesis was carried out using PrimeScript reverse transcriptase (TaKaRa).

For real-time PCR, the first strand cDNA was synthesized using PrimeScript Reverse Transcriptase (TaKaRa, Japan).

24 Briefly, whole cellular RNA was extracted, and reverse transcription was performed using PrimeScript Reverse Transcriptase(TaKaRa Biotechnology, Dalian, China).

Approximately 0.5  μg of the extracted RNA was reverse-transcribed into cDNA using Primescript reverse transcription (RT) Master Mix (TaKaRa Biotechnology Co., Dalian, China).

Total RNA was then directly used for first-strand cDNA synthesis using PrimeScript Reverse Transcriptase (TaKaRa) and the oligo dT) primer 5′-ATTCTAGAGGCCGAGGCGGCCGACATGT(18 VN-3′.

We used PrimeScript reverse transcriptase (Takara Biotechnology, Dalian, China) and oligo dT) to synthesize the first-strand cDNA according to the manufacturer's instructions.