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By analyzing gill tissues for CEV in seven out of eleven samples by a nested PCR, PCR products of 547 bp and 180 bp (by using nested primers) could be amplified.
By using Nested, both are guaranteed.
Changes in the composition of AOB were examined by using nested PCR, denaturing gradient gel electrophoresis (DGGE) and sequencing of DGGE bands.
In [39], by using nested lattice codes, the generalized degrees of freedom for the two-user cognitive interference channel are characterized where one of the transmitters has knowledge of a linear combination of the two information messages.
Surveys of ground vegetation were carried out by using nested quadrats (plots of 0.25 and 0.016 m2 size) located along normally five transects running perpendicular to the habitat boundaries.
Viral DNA was detected by using nested PCR in 59 out of 92 bovine blood samples (64.1%), and by qPCR in 68 out of 92 bovine blood samples (73.9%), but none out of 69 DNA samples isolated from wild ruminant blood clots, was positive in both assays.
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In addition, 11 HBoV sequences were amplified by using nested-PCR for HBoV2.
In the second step, the cell-specific promoter and the gene fragment were fused by amplification using nested primers.
Detectable fragments were excised from the gel and reamplified by PCR using Nested Primer 1 and Nested Primer 2R included in the subtraction kit.
Briefly, the recombinant clones including cDNA inserts were amplified by PCR using nested PCR primers with Pfu DNA polymerase (Promega, Madison, Wl, USA).
The 5' end of AnoACE7 was determined by RT-PCR analysis using nested primers designed against the genomic sequence.
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