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Its small, sequenced and well-annotated genome and the efficient gene deletion techniques allowed the production of a collection of complete ORF deletion mutants where each deletion mutant is tagged by two unique barcodes [ 2].
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This approach takes advantage of the two unique barcode sequences associated with each deletion mutant, called uptag and downtag (Kim et al. 2010).
Four unique forward barcodes (F-barcode) and eight unique reverse barcodes (R-barcode) were used to code the 32 samples.
After sequencing, the reads are deconvoluted by their unique barcodes for downstream analysis.
The questionnaires and electronic database were linked by these unique barcodes that were kept separately in a password protected database.
In one experiment (identified as experiment 2 in Table S1), ten different samples were distinguished by adding unique "barcode -labeled amplification primers (provided as part of the SOLiD SREK kit).
The copy number is determined by the number of unique barcodes instead of the number of reads.
First, the reads are separated by amplicon, and the unique barcodes in each amplicon are ordered according to the number of reads containing the barcode.
Identifications were mainly in agreement, but the presence of an unknown trypanosome in several samples was revealed by its unique barcode.
Samples of these different types of chemical compounds can be associated with a container identified by a unique barcode.
Samples and data are identifiable by a unique barcode only, and volunteers are free to withdraw from these projects at any time.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com