The crude laccase extract was further purified by three successive steps of ion exchange chromatography: firstly on DEAE-cellulose (10 mM phosphate buffer, pH 7.0) with a flow rate of 2 mL/min, secondly on CM-cellulose (10 mM phosphate buffer, pH 6.6) with a flow rate of 2 mL/min, and finally on Q-Sepharose (10 mM sodium acetate buffer, pH 4.0) with a flow rate of 1 mL/min.
Since the early stage of the polyolefins development, properties have been expanded by improving catalyst chemistry, morphology and by conducting polymerization in two successive steps, generating in each step a different polymer in terms of molecular weight, crystallinity, composition to match the designed properties balance.
Chitin, found in the cuticle of the integument and peritrophic membrane of the midgut, is a linear polymer of β-1,4 linked N-acetylglucosamine. Chitin degradation is catalyzed by chitinolytic enzymes in two successive steps: chitinase (EC 3.2.1.14) hydrolizes chitin into oligosaccharides of GlcNAc, then β-N-acetylhexosaminidase further degrades the oligomers into monomers.
The Firmenich researchers show that the formation of sclareol is catalysed in two successive steps by two different enzymes.
The statistical significance of a difference between point estimates was judged in two successive steps by first examining whether either confidence interval contained the other point estimate.
Accordingly, we introduce the concept of a reaction module to define at least two successive steps catalyzed by homologous enzymes in pathways alignable by similar chemical reactions.
Instead of requiring two successive steps ('denoising' followed by clustering), we have shown here that the clustering process itself can be used at the same time to produce a robust partition (insensitive to sequencing errors) and to identify the outlier sequences, i.e. those that were pooled as 'noise' in this article.
The formation of a char has been considered as having two successive steps: a multibubble stage, followed by a single-bubble stage.
GABA is catabolized in mitochondria through the GABA-shunt [2], a metabolic pathway that bypasses two successive steps of the tricarboxylic acid cycle catalyzed by α-ketoglutarate dehydrogenase and succinyl CoA ligase.
