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Immunostaining on the TMAs was graded semi-quantitatively considering both staining intensity and percentage of positive tumor cells by two study pathologists (S.J.J. and M.J.K).
Pimonidazole scoring was performed independently by two study pathologists (LV and AKL).
The grade for all cancers was reviewed by two study pathologists (CSL, JRK).
Per biopsy, tumour histology was independently verified by two study pathologists (CF and BL).
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Any cases with different classification of immunostaining patterns were reviewed simultaneously by three study pathologists, using a multi-headed microscope.
Biopsy slides were independently reviewed by one of two study pathologists (SJS, JLC) who were blinded to the participants' case or control status.
Cancer diagnoses for all patients were confirmed by two senior study pathologists by review of tumor slides.
Histopathological interpretation of biopsies is conducted at two laboratories by study pathologists who are blinded to the screening results that led the patient to colposcopy.
A subset of 11 tumors were removed from further analysis based on neuroendocrine features following pathological review of all tumors by one of the study pathologists (W. Travis).
Development of HCC was studied by two independent pathologists who were blind to the study.
Thirty-three cases were identified, and the slides from each case were reviewed by the study pathologists (H.Z.K. and A.B.S).
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