Sentence examples for by two steps first from inspiring English sources

The mechanism is characterised by two steps: first, a low temperature particle growth and second, a high temperature annealing.

The formation process of microspheres was achieved by two steps: first, after droplets dropped off the delivery tube, they became approximately spherical in the air under the effect of the surface tension; second, droplets dropped into liquid condensate and maintained the spherical shape, and were frozen during the free settling process.

The procedure is based on a global approach strategy, composed by two steps: first, the response of the panel is obtained by a neural network system using the results of finite element analyses and, in a second step, a multi-objective optimization problem is solved using a genetic algorithm.

Slums in the cities were identified by two steps, first by using the satellite images and secondly by ground truthing.

A diploid Ndt80-myc sum1Δ deletion strain was made by two steps: first, the SUM1 ORF was replaced with KANMx in a haploid W303 Ndt80-myc strain, using homologous-recombination based transformation [ 42].

MCI was defined by two steps: first, subjects with post-stroke disease, Alzheimer's disease or Parkinson's disease and MMSE< 18 were excluded; and then subjects were categorized as MCI MMSEE scores between 19 and 24) and normal (MMSE scores between 25 and 30).

The raw data were modified by following two steps: first, the adapter and index sequences in reads were deleted, and then, the reads that contained more than 50%% low-quality bases (quality value ≤ 5) were removed.

This investigation considers the fluctuation of cutting forces caused by cutter vibration in two steps: First, a cutting force model that is combined with dynamic undeformed chip thickness is built for bull-end milling.

In contrast, the major strategy for surface functionalization by biological molecules includes two steps, first synthesizing the small molecules or polymers functionalized NPs, and then coupled to the biomolecules by chemical bond or physical adsorption.

Antibodies raised against Msi1 were affinity-purified from the rabbit antisera by column chromatography in two steps: first using the antigen (GST-Msi1 protein) and then using the GST purified protein.

The cassette used for hacA deletion was produced by fusion-PCR in two steps: first, independent amplification of the hacA promoter and terminator regions (each ≅ 550 bp) and the AopyrG gene, respectively, using primers summarised in Table  2.