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Amidation is catalysed starting from a glycine-extended prohormone, by two sequential actions of two enzymes, peptidylglycine α-hydroxylating monooxygenase and peptydilamido-glycolat lyase.
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In this sense, in the present work we attempted to evaluate the beneficial effects of exogenous NO and its levels of action (biochemical and cellular) in a model of SIRS induced by two sequential insults.
This oxidation is brought about by the enzyme by two sequential one-electron transfers (Veitch 2004).
Membranes were obtained by two sequential centrifugations.
Genotyping were performed by two sequential PCR, which products were analyzed by sequencing.
The NaTrxhΔNβ cDNA with Bam-HI and Eco-RI sites was generated by two sequential PCRs.
BACs were modified by two sequential steps of retrofitting using the lambda recombination system [ 63].
In eukaryotic cells, efficient lesion bypass is achieved cooperatively by two sequential steps.
Four sequential action research cycles built upon baseline data including 614 acute hip-fracture inpatients and 30 purposefully sampled clinicians.
The proteasomes were isolated by three sequential centrifugations as described previously [ 31- 33].
Ca2+ loading was started by three sequential additions of 20 μM CaCl2.
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