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The trees identified Leishmania tropica from 16 isolates which were represented by two sequence types.
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eDouble sequence indicating two sequence types within the same BES.
aDouble sequence in the centre indicating two sequence types.
Genotyping of 47 strains by 16S rRNA gene sequencing resulted in nine sequence types, whereas PFGE after digestion with SmaI resulted in 22 subtypes.
Thirty sequence types were found once; the remaining 23 were represented by up to 11 accessions.
MLST typing identified eight sequence type (ST) groups.
Of these, 92 amplicons were successfully sequenced and represented by two distinct sequence types [GenBank accession numbers KF204571 and KF204572].
The BchD/ChlD phylogenetic tree in figure 3 C was rooted by two sequences of von Willebrand factor Type A, which is considered to be an ancient protein domain (Ponting et al. 1999).
This inference is supported by the pattern observed for the four nLSU sequence types of A. papernai, which are all present in Madagascar.
Thirty different sequence types (STs) were identified by using MST.
Multi-locus sequence typing of the 39 isolates resolved three unique sequence types.
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