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Each slide was independently evaluated by two pathologists unaware of clinicopathological characteristics of the tumor.
To assay cellular apoptosis, terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) staining was performed by two pathologists unaware of study group allocation.
Pathological diagnosis were performed by two pathologists, unaware of the genetic information, of Kyoto University Hospital Laboratory of Anatomic Pathology, and finally confirmed by one pathologist (TM) according to the WHO classification system (Travis et al, 1999).
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The staining was evaluated independently by two pathologists, who were unaware of patient survival.
IHC staining was evaluated by two pathologists who were unaware of the patients' clinical information.
Tumor samples were diagnosed in accordance with World Health Organization (WHO) system, by two pathologists who were unaware of patient data.
PMSD1-expression analysis was performed by two pathologists who were unaware of the findings of the clinical data.
PR and ER nuclear and cytoplasmic expression was jointly scored by two pathologists (Maria Guido and Kathrin Ludwig) unaware of the patients' clinical history.
Semiquantitative assessment of the ISH staining results was performed by two pathologists (CKJ and SHL) who were unaware of the clinicopathological and immunohistochemical data.
Histopathological studies were performed by two pathologists (LRB and MG) experts in liver diseases, who were unaware of patients' characteristics and outcome.
The stained slides were observed and diagnosed by two pathologists, respectively, under an optical microscope.
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