Sentence examples for by two passes through from inspiring English sources

The cells were broken by two passes through a French press at 950 p.s.i. in buffer A200 (25 mM HEPES pH 7.4, 200 mM KCL, 10% (w/v) glycerol and 2 mM β-mercaptoethanol) containing 4% Triton, complete protease inhibitors (Roche) and 2 mM PMSF.

Cells were lysed by two passes through a French press.

Cells were then lysed by two passes through a French press at 1000 psi.

Cell lysis was effected by two passes through a French pressure cell at 14000 psi.

Cells were lysed by two passes through a French Press (SIM Aminco) at 1000 p.s.i. and the crude extract clarified by centrifugation (45 mins, 35000 r.p.m).

Cells were lysed by two passes through a microfluidizer (M-110Y) at 15000 psi, and all subsequent steps were conducted at 4 °C.

The Black Dog Pub housed a smoking bar connected by two pass-through windows and two open doorways to a nonsmoking dining room.

Cells were broken by three passes through a French press at 15 kpsi.

Cells were lysed by three passes through a pre-cooled small French Press cell (Thermo IEC, Waltham, MA, USA) at 12,500 psi.

Cold cell lysate was homogenized by five passes through a 28-gauge needle and clarified by centrifugation at 25,000 x g for 15 min. EZview Red ANTI-FLAG M2 affinity gel (60 µl settled resin; Sigma-Aldrich) was added to 500 µg of lysate protein in 500 µl lysis buffer and rotated overnight at 4°C.

Cells were disrupted by homogenisation followed by five passes through a 25 gauge needle.