Sentence examples for by two passages through from inspiring English sources

Cells were disrupted by two passages through a French press.

Next, cells were disrupted by two passages through a French Press (2500 PSI).

Cells were disrupted by two passages through a French pressure cell at 15,000 psi and cellular debris removed by centrifugation.

Bacteria were suspended in PBS, 0.05% (v/v) Tween 20, 2 mM EDTA, 0.5 mM PMSF and 0.1% (v/v) β-mercaptoethanol and lysed by two passages through a French press (Spectronics Instruments Inc).

The cells were broken by two passages through a French Press at 20,000 psi and unbroken cells and cell debris were removed by centrifugation at 3,000 x g at 4°C.

Cell disruption was performed by two passages through a cell disruptor (Constant Systems Ltd) at 39 kPsi in the presence of 1 mM MgSO4, 1 mM PMSF, 100 µg/mL DNase and 100 µg/mL RNase.

After incubation for 30 min on ice platelets were lysed by five passages through an insulin syringe needle.

For each mouse, the brain was dissected, weighed, homogenized by three passages through a 20 gauge syringe in 2 ml of 1 mg/ml collagenase/dispase.

50 biotinylated oocytes were homogenized in 1 ml lysis buffer by seven passages through a 20 gauge needle and three passages through a 27 gauge needle, with 10 µl Protease Inhibitor cocktail (Sigma) added to protect proteins from digestion.

Cells were harvested, resuspended in 50 mM Tris (pH 8.5), 50 mM KCl, 5 mM MgAc, 0.1% NaN3 and 300 µM PMSF, and lysed by three passages through a French cell press.

For analysis of membrane-bound proteins (retGC, GNAT2, cone opsins) membrane fractions were prepared by homogenising tissue in low salt homogenization buffer (10 mM MOPS pH 7.3, 5 mM mercaptoethanol, 20 µg/ml leupeptin, 1 mM PMSF) and the cells left to swell on ice for 10 min. The cells were lysed by five passages through a 0.5 mm gauge syringe needle.