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Gene knockdown (RNAi) was induced by two injections of 15 μg of dsRNA into fourth instar nymphs.
Single point immunization with admixtures of PLA microparticles and alum resulted in antibody response very close to that achieved by two injections of alum-adsorbed TT; the antibody titers were more than 50 μg/ml over a period of 6 months.
To this end, animals were sequentially exposed to DENA and RS, followed by two injections of hepatocytes freshly isolated from normal syngenic donors [ 14].
To achieve comparable hormonal conditions, cows were synchronized by two injections of -cloprostenol (Dalmazin®, Selectavet, Weyarn, Germany) at 12-day intervals.
We used the model of pain memory described by Igor Kissin [ 3], in which inflammation was induced by two injections of carrageenan.
In comparison with other syngeneic tumors, the regression of implanted E.G7-OVA in 33D1+ DC-deleted mice was almost unchanged by two injections of LPS.
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The system suitability was determined by six injections of teneligliptin (300 μg/mL).
The precision of LOD and LOQvalues were experimentally verified by six injections of standard solutions of thecompounds at the determined concentrations.
EndoPTP1B−/− or wild type (WT) mice received LPS (1 mg/kg) or saline solution followed by five injections of glucose (2 g/kg) or saline solution each hour 12 h after LPS.
Carryover was evaluated by three injections of an upper limit of quantification (ULOQ) sample, immediately followed by three injections of a blank plasma sample.
The first protocol consisted of three therapeutic immunisations with LM-Mb followed by three injections of αGC.
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