Exact(1)
Guanosine triphosphate cyclohydrolase I (GTPCH; EC 3.5.4.16) catalyzes the formation of dihydroneopterin triphosphate from GTP, and BH4 is generated by two further steps through 6-pyruvoyltetrahydropterin synthase and sepiapterin reductase.
Similar(59)
In particular, recent articles by Bishop and Shames [61, 62] are two further steps in developing computationally efficient algorithms that extend the existing results for globally rigid networks in the context of easily localizable networks where distance and bearing measurements are noisy.
Two further steps are performed by a single protein, BAG-1, another co-chaperone of Hsp70.
The challenge requires two further steps, I think.
Two further extraction steps by adding 1 volume of 37% formaldehyde into 2 volume re- suspended cells in PBS were applied.
They might perhaps be expanded with a sentence or two about further steps suggested by the material reported on in this paper and/or by the general approach of the group that have contributed to this line of research.
Fractions 4 and 5 (400 mg) were further purified by two chromatography steps on Merck silica gel (230 400 mesh, ATSM) using CHCl3:MeOH:H2O in increasing polarity.
The generalized NFM is further enhanced by two online step control processes, namely, the Convex Step-size Control (CSC) process and the Hyper-Plane Step Control (HPSC) process.
Subsequently, the cyclized OPDA is transferred from chloroplasts into peroxisomes, where it is reduced by OPDA reductase3 (OPR3) and three further β-oxidation steps are conducted to produce (3R, 7S --JA.
The crude laccase extract was further purified by three successive steps of ion exchange chromatography: firstly on DEAE-cellulose (10 mM phosphate buffer, pH 7.0) with a flow rate of 2 mL/min, secondly on CM-cellulose (10 mM phosphate buffer, pH 6.6) with a flow rate of 2 mL/min, and finally on Q-Sepharose (10 mM sodium acetate buffer, pH 4.0) with a flow rate of 1 mL/min.
Within the chancel, the sanctuary is raised by a further three steps and marked by a rail.
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